We are proud to announce that Yadilette Rivera-Colon is one of nine winners of the Women of Color STEM Achievement Awards foe 2021. See the full announcement here.
Yadilette completed her PhD in the Garman Lab in 2013, and is now an Assistant Professor of Biology at Bay Path University.
UGGT1 and UGGT2 are key quality control factors that determine the fate of glycoproteins in the early mammalian secretory pathway. These two paralogues direct persistent molecular chaperone binding in the endoplasmic reticulum that helps with protein maturation and sorting. Persistent chaperone binding of terminally misfolded clients can target proteins for degradation by the proteasome, as well as lysosomal proteases. In our study, a quantitative glycoproteomics strategy was developed to identify cellular clients of UGGT1 and UGGT2. Interestingly, UGGT1 was found to preferentially recognize large membrane glycoproteins, while UGGT2 favored the modification of smaller soluble lysosomal proteins. This study opens the door to a detailed understanding of the recognition process for these important quality control factors and identifies targets whose folding trajectories may be altered in disease states. This work, recently published in eLife, blends chemical and cell biological approaches and was a collaborative effort by three CBI students in the lab, spearheaded by lead author Ben Adams. Ben recently started a postdoctoral position at Harvard Medical School. – Dan Hebert
“Our work identified native substrates of an essential protein quality control process and explored it’s role in glycoproteostasis.” – Ben Adams
“Novel substrates of the glucosyltransferases UGGT1 and UGGT2 have been identified and compared to an in silico N-glycoproteome” – Nathan Canniff
“Through a quantitative mass spectrometry approach we have determined endogenous substrates for UDP-glucose:glycoprotein glucosyltransferases (UGGT) 1 & 2, showing both are functional and preferentially target different substrates requiring folding assistance from calnexin and calreticulin” – Kevin Guay
Quantitative glycoproteomics reveals cellular substrate selectivity of the ER protein quality control sensors UGGT1 and UGGT2.Adams BM, Canniff NP, Guay KP, Larsen ISB, Hebert DN. Elife. 2020 Dec 15;9:e63997. doi: 10.7554/eLife.63997.PMID: 33320095. Free PMC article
Co-first author Dougan adds, “While cavitation is often thought of as something to be avoided, we aim to use it to benefit medicine and the development of new treatments.” For example, cavitation rheology can be used to measure the strength of interfaces within the brain, which is difficult to achieve with any other method, she notes. Specifically for TBI, the authors outline techniques for biologists to establish cavitation rheology as a tool for characterizing mechanical responses of soft biological tissues.
Read more from the press release by Al Crosby, Professor of PSE
CBI Trainee Weiyue Xin (Santore Lab) took third place in the Annual G.R.A.S.S. (Graduate Research Student Symposium) for her seminar on the interactions between solid domains in biomimetic membranes. Weiyue’s work is part of a larger DOE-sponsored collaboration between the Santore and Grason groups, aiming to understand new mechanisms occurring in membranes made from biomolecules, and how these interactions can be exploited in the creation of new materials.
What a happy coincidence that on the day of the announcement that Jennifer Doudna and Emmanuelle Charpentier were awarded the Nobel Prize in Chemistry for basic science at the interface of Chemistry and Biology, CBI’s monthly Chalk Talk featured the labs of two women, Amanda Woerman and Jeanne Hardy. Jeanne took a moment to mark the historic occasion, expressing both the happiness felt by many who are “really thrilled to be doing science at a time when women are able to do great science and be recognized,” and also encouragement to the entire community to continue to find ways to make science welcoming to everyone.
CBI Trainee Adrian Lorenzana won first prize in the Materials Science and Engineering category in the NOBCChE ConneXions Poster Competition 2020 for his poster presentation “Force-responsive Materials Utilizing Cryptic Crosslinking Sites.” Congratulations Adrian!
October 23, 2020, 10am-2pm
Join us for this online event to celebrate the 25th birthday of the CBI Program!
Featuring CBI Alumni leading Career Panel Discussions.
Showcasing current CBI student research in the new IALS Core Facilities.
Lila’s research focus for decades has been protein folding, that is, how amino acid sequence determines the three-dimensional structure of a protein. She is particularly focused on how proteins fold in the cellular environment and the role of molecular chaperones in ensuring high fidelity in the folding process.
Lila says of her selection, “I am thrilled by this honor. The recognition of one’s contributions over a career by colleagues is truly gratifying.”
The National Academy of Sciences (NAS) is a private, non-profit society of distinguished scholars established by an Act of Congress signed by President Abraham Lincoln in 1863. The academy is charged with providing independent, objective advice to the nation on matters related to science and technology. Scientists are elected by their peers to membership in the NAS for outstanding contributions to research.
NAS is committed to furthering science in America, and its members are active contributors to the international scientific community. Nearly 500 members of the NAS have won Nobel Prizes. This year’s group, which also includes 25 non-voting associate members, citizens of foreign countries, brings the total number of active members to 2,347 and the total number of foreign associates to 487. The society’s journal, Proceedings of the National Academy of Sciences, founded in 1914, is one of the premier international journals publishing results of original research.
In 2018, Lila received the American Chemical Society’s Ralph F. Hirschmann Award in Peptide Chemistry for “her seminal contributions to peptide structure and function, peptide models for protein folding and function, and roles of peptide and protein aggregation in disease.” In 2016, she was elected to the American Academy of Arts and Sciences. That same year, the American Society for Biochemistry and Molecular Biology named her editor in chief of the Journal of Biological Chemistry, the society’s flagship journal, for a five-year term. In 2014, she was named to the National Institutes of Health Council of Councils, established to advise the NIH director on policies and activities of the Division of Program Coordination, Planning and Strategic Initiatives, which includes making recommendations on research that represents important areas of emerging scientific opportunities, rising public health challenges or knowledge gaps that deserve special emphasis or would otherwise benefit from strategic planning and coordination.